Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T47D breast cancer cell line
cell line
T47D
cell type
ERalpha-positive breast cancer cells
chip antibody
ERalpha (H-20, Santa Cruz)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and ERα complexes were isolated with antibody. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols

Sequencing Platform

instrument_model
Illumina Genome Analyzer

hg19

Number of total reads
47674108
Reads aligned (%)
72.7
Duplicates removed (%)
25.5
Number of peaks
7910 (qval < 1E-05)

hg38

Number of total reads
47674108
Reads aligned (%)
75.3
Duplicates removed (%)
23.5
Number of peaks
8061 (qval < 1E-05)

Base call quality data from DBCLS SRA