Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Lung
Cell type
IMR-90
Primary Tissue
Lung
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
immortalized fetal lung fibroblasts
experiment
ChIP-seq (INPUT)
cell line
IMR90hTert
passage
6-13
treatment
Cells treated with #1MAF1 siRNA (Ambion 427037, id s38693), 36hrs; then serum starved (8 hrs)
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChiP-seq was performed as described (Canella et al., 2010) Libraries were prepared with 12 ng of ChIP DNA (ChIP-seq) or genomic DNA (INPUT) according to manufacturer's's instruction with the ChIP-seq DNA Sample prep kit” (Illumina, 1003473)

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg38

Number of total reads
223564107
Reads aligned (%)
92.4
Duplicates removed (%)
17.2
Number of peaks
7250 (qval < 1E-05)

hg19

Number of total reads
223564107
Reads aligned (%)
91.6
Duplicates removed (%)
18.8
Number of peaks
3952 (qval < 1E-05)

Base call quality data from DBCLS SRA