Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Lung

Cell type

IMR-90

Primary Tissue

Lung

Tissue Diagnosis

Normal

Sample

source_name

immortalized fetal lung fibroblasts

experiment

ChIP-seq (INPUT)

cell line

IMR90hTert

passage

6-13

treatment

Cells serum starved (8 hrs) + 10 µM Insulin (90 min)

chip antibody

none

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChiP-seq was performed as described (Canella et al., 2010) Libraries were prepared with 12 ng of ChIP DNA (ChIP-seq) or genomic DNA (INPUT) according to manufacturer's's instruction with the ChIP-seq DNA Sample prep kit” (Illumina, 1003473)

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

169310836

Reads aligned (%)

85.3

Duplicates removed (%)

63.4

Number of peaks

7544 (qval < 1E-05)

hg19

Number of total reads

169310836

Reads aligned (%)

84.7

Duplicates removed (%)

64.8

Number of peaks

6096 (qval < 1E-05)