Antigen

Antigen Class

RNA polymerase

Antigen

RNA polymerase II

Cell type

Cell type Class

Blood

Cell type

THP-1

Primary Tissue

Blood

Tissue Diagnosis

Leukemia Acute Myelogenous

Sample

source_name

THP1, pLKO.1-PAF1 shRNA #1

cell line

THP1

genotype/variation

PAF1 knockdown

chip antibody

Pol II (ser-5p) (Abcam, ab5131)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

ChIP-seq libraries were constructed with 5 to 10 ng immunoprecipitated DNA. Samples were sequenced on HiSeq 2000 by following the manufactor's protocols. RNA libraries were prepared for sequencing using strand-specific protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

40433058

Reads aligned (%)

90.9

Duplicates removed (%)

10.7

Number of peaks

29063 (qval < 1E-05)

hg19

Number of total reads

40433058

Reads aligned (%)

90.2

Duplicates removed (%)

11.4

Number of peaks

28776 (qval < 1E-05)