Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
Th17 Cells
MeSH Description
A subset of helper-effector T-lymphocytes which synthesize and secrete INTERLEUKINS IL-17; IL-17F; and IL-22. These cytokines are involved in host defenses and tissue inflammation in autoimmune diseases.

Attributes by original data submitter

Sample

source_name
In vitro Th17
strain
C57BL/6
cell type
T cell
age
6-8wks
genotype
DDX5fl/fl, CD4Cre+
chip antibody
N/A
chip antibody vendor
N/A

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Total and IP enriched sonicated chromatin from cultured Th17 cells were harvested using Qiagen PCR purification kit. ChIP-seq libraries were prepared for sequencing using standard Illumina protocols. 1% PhiX spiked in during the sequencing process and that allows us to calculate QC metrics for each lane and determine if poor performance is library or sequencer related.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
26449323
Reads aligned (%)
99.6
Duplicates removed (%)
8.6
Number of peaks
80 (qval < 1E-05)

mm9

Number of total reads
26449323
Reads aligned (%)
99.6
Duplicates removed (%)
9.0
Number of peaks
315 (qval < 1E-05)

Base call quality data from DBCLS SRA