Antigen

Antigen Class

TFs and others

Antigen

EBNA1

Cell type

Cell type Class

Others

Cell type

C666-1

NA

NA

Sample

source_name

EBNA1 CHIP-seq in C6661

cell line

C6661

cell type

EBV positive nasopharyngeal carcinoma (NPC) line

chip antibody

EBNA1

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lu et al, 2010 Virology journal 7, 262 The EBNA1 antibody is a rabbit polyclonal antibody raised to full length EBNA1(lacking Gly-Ala repeats). The rabbit injections and bleeds were provided by Pocono Rabbit Farm. Reactive serum was affinity purified against full length EBNA1, and 10 ug of affinity purified antibody was used for 50 million cells for each ChIP experiment. TruSeq ChIP Sample Prep kit ChIP-Seq protocol is as described previously (Lu et al, 2010 Virology journal 7, 262). Total RNA was isolated for RNA-Seq using Trizol reagent.

Sequencing Platform

instrument_model

Illumina Genome Analyzer II

hg38

Number of total reads

15235443

Reads aligned (%)

91.9

Duplicates removed (%)

5.7

Number of peaks

1053 (qval < 1E-05)

hg19

Number of total reads

15235443

Reads aligned (%)

90.9

Duplicates removed (%)

6.4

Number of peaks

1413 (qval < 1E-05)