GSM1905008: EBNA1 CHIP-seq in MutuI; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
EBNA1
Cell type
Cell type Class
Blood
Cell type
Mutu
NA
NA
Attributes by original data submitter
Sample
source_name
EBNA1 CHIP-seq in MutuI
cell line
MutuI
cell type
EBV positive B-cell
chip antibody
EBNA1
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lu et al, 2010 Virology journal 7, 262 The EBNA1 antibody is a rabbit polyclonal antibody raised to full length EBNA1(lacking Gly-Ala repeats). The rabbit injections and bleeds were provided by Pocono Rabbit Farm. Reactive serum was affinity purified against full length EBNA1, and 10 ug of affinity purified antibody was used for 50 million cells for each ChIP experiment. TruSeq ChIP Sample Prep kit ChIP-Seq protocol is as described previously (Lu et al, 2010 Virology journal 7, 262). Total RNA was isolated for RNA-Seq using Trizol reagent.