Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic kidney
NA
NA

Attributes by original data submitter

Sample

source_name
mouse embryonic kidney
developmental stage
16.5 days
strain
Swiss Webster
chip antibody
NA

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cortex of human embryonic kidneys were micro-dissected and fixed in crosslinking buffer containing 1% PFA for 30 min, then homogenized, followed by chromatin extraction and sonication. Sonicated chromatin was then immuno-precipitated with the indicated antibodies. The precipitated DNA was subsequently collected with Qiagen PCR product purification kit. Mouse embryonic kidneys were processed in a similar way. Libraries were constructed using ThruPLEX-FD Prep Kit (Rubicon Genomics), and subsequently sequenced by Illumina HiSeq 2000.

Sequencing Platform

instrument_model
NextSeq 500

mm10

Number of total reads
60494657
Reads aligned (%)
97.4
Duplicates removed (%)
21.3
Number of peaks
394 (qval < 1E-05)

mm9

Number of total reads
60494657
Reads aligned (%)
97.1
Duplicates removed (%)
21.2
Number of peaks
433 (qval < 1E-05)

Base call quality data from DBCLS SRA