Antigen

Antigen Class

TFs and others

Antigen

SIX2

Cell type

Cell type Class

Kidney

Cell type

Kidney Cortex

MeSH Description

The outer zone of the KIDNEY, beneath the capsule, consisting of KIDNEY GLOMERULUS; KIDNEY TUBULES, DISTAL; and KIDNEY TUBULES, PROXIMAL.

Sample

source_name

human embryonic kidney cortex

developmental stage

17 weeks

strain

NA

chip antibody

SIX2 antibody (MyBiosource, MBS610128)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cortex of human embryonic kidneys were micro-dissected and fixed in crosslinking buffer containing 1% PFA for 30 min, then homogenized, followed by chromatin extraction and sonication. Sonicated chromatin was then immuno-precipitated with the indicated antibodies. The precipitated DNA was subsequently collected with Qiagen PCR product purification kit. Mouse embryonic kidneys were processed in a similar way. Libraries were constructed using ThruPLEX-FD Prep Kit (Rubicon Genomics), and subsequently sequenced by Illumina HiSeq 2000.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

30440545

Reads aligned (%)

97.5

Duplicates removed (%)

5.1

Number of peaks

47131 (qval < 1E-05)

hg19

Number of total reads

30440545

Reads aligned (%)

97.1

Duplicates removed (%)

5.4

Number of peaks

47070 (qval < 1E-05)