Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryo
Cell type
Embryonic brains
NA
NA

Attributes by original data submitter

Sample

source_name
control_E13.5_cortex_input
strain background
C57BL6/J
developmental stage
E13.5
cre expression
Emx1-Cre
genotype/variation
BAF155fl/+, BAF170fl/+
sample group
Control
tissue
cortex

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Murine embryonic cortices were homogenized in sucrose solution and fixed with 37% formaldehyde. In subsequent steps histone-DNA comlexes were isolated with antibody. Library preparation was performed according to standard protocols of Illumina (TruSeq, Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
24774787
Reads aligned (%)
98.7
Duplicates removed (%)
8.9
Number of peaks
305 (qval < 1E-05)

mm9

Number of total reads
24774787
Reads aligned (%)
98.5
Duplicates removed (%)
8.9
Number of peaks
319 (qval < 1E-05)

Base call quality data from DBCLS SRA