Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Neural
Cell type
Cerebellar Cortex
MeSH Description
The superficial GRAY MATTER of the CEREBELLUM. It consists of two main layers, the stratum moleculare and the stratum granulosum.

Attributes by original data submitter

Sample

source_name
dcKO_P1_cortex_input
strain background
C57BL6/J
developmental stage
P1
cre expression
Nex-Cre
genotype/variation
BAF155fl/fl, BAF170fl/fl
sample group
conditional double knock-out (dcKO) of BAF155/BAF170 complex (dcKO)
tissue
cortex

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Murine embryonic cortices were homogenized in sucrose solution and fixed with 37% formaldehyde. In subsequent steps histone-DNA comlexes were isolated with antibody. Library preparation was performed according to standard protocols of Illumina (TruSeq, Illumina).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
24902046
Reads aligned (%)
98.7
Duplicates removed (%)
10.2
Number of peaks
294 (qval < 1E-05)

mm9

Number of total reads
24902046
Reads aligned (%)
98.5
Duplicates removed (%)
10.2
Number of peaks
322 (qval < 1E-05)

Base call quality data from DBCLS SRA