Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Cardiovascular
Cell type
Heart Ventricles
MeSH Description
The lower right and left chambers of the heart. The right ventricle pumps venous BLOOD into the LUNGS and the left ventricle pumps oxygenated blood into the systemic arterial circulation.

Attributes by original data submitter

Sample

source_name
input for all P14 samples
strain background
C57BL/6
genotype/variation
Myh6-Cre+ ; Eed fl/fl (EED Knockout; CKO)
tissue
Heart
cell type
isolated adult ventricular cardiomyocytes
chip antibody
none (input)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Fragments 150-300 bp were size-selected by 2% agarose gel electrophoresis. Recovered DNA was amplified using Phusion DNA polysome (NEB), multiplexing PCR primer 1.0, and one indexed primer. The amplified libraries were purified with Agencourt AMPure XP beads (Beckman Coulter). The libraries were quantitated using the Quant-iT DNA quantitation kit (Invitrogen). The DNA size distribution of the library was measured by an Agilent Bioanalyzer. ChIP-seq library preparation were performed according to the instruction of NEB NEXT-generation library preparation kit. 2 independent ChIP samples were used for one single ChIP-seq library preparation

Sequencing Platform

instrument_model
Illumina HiSeq 2500

mm10

Number of total reads
37704818
Reads aligned (%)
97.8
Duplicates removed (%)
16.6
Number of peaks
569 (qval < 1E-05)

mm9

Number of total reads
37704818
Reads aligned (%)
97.5
Duplicates removed (%)
16.6
Number of peaks
616 (qval < 1E-05)

Base call quality data from DBCLS SRA