Protocol was followed esentially as described previously (Schwartz, B.E., Larochelle, S., Suter, B., and Lis, J.T. 2003 Mol Cell Biol). Briefly, crude nuclei were isolated and sonicated to aquire an average fragment size ~300 bp followed by IP using rat-anti-dHNF4 antibody and protein G dynabeads (Life Technologies) Libraries were prepared using NEB ChIPseq library prep kit