Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Hnf4

Cell type

Cell type Class
Adult
Cell type
Adult body
NA
NA

Attributes by original data submitter

Sample

source_name
mautre adults_whole animal_males + females_dHNF4 ChIP
strain background
w1118
developmental stage
mature adults
tissue
whole animal
gender
mixed
chip antibody
rat anti-dHNF4 3600 (PMID 19254568)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Protocol was followed esentially as described previously (Schwartz, B.E., Larochelle, S., Suter, B., and Lis, J.T. 2003 Mol Cell Biol). Briefly, crude nuclei were isolated and sonicated to aquire an average fragment size ~300 bp followed by IP using rat-anti-dHNF4 antibody and protein G dynabeads (Life Technologies) Libraries were prepared using NEB ChIPseq library prep kit

Sequencing Platform

instrument_model
Illumina HiSeq 2000

dm3

Number of total reads
24483693
Reads aligned (%)
82.5
Duplicates removed (%)
11.3
Number of peaks
0 (qval < 1E-05)

dm6

Number of total reads
24483693
Reads aligned (%)
78.7
Duplicates removed (%)
14.8
Number of peaks
2641 (qval < 1E-05)

Base call quality data from DBCLS SRA