Antigen

Antigen Class

Histone

Antigen

H2A.V

Cell type

Cell type Class

Cell line

Cell type

Kc167

Source

e/se

Developmental Stage

dorsal closure stage

Sample

source_name

Kc167

experiment

?-H2Av

chip antibody

Mouse anti-?-H2Av (DSHB UNC93-5.2.1-s)

RNAi treatment

PR-Set7

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In early origin experiments, chromatin was isolated and BrdU was immunoprecipted as described in (Ryba et al., Nat Protoc, 2011). H4K20me1 and γ-H2Av ChIP were performed as described in (MacAlpine et al., Genome Research, 2010). Sequencing libraries were prepared using Illumina's TruSeq protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

14057618

Reads aligned (%)

95.6

Duplicates removed (%)

14.3

Number of peaks

2667 (qval < 1E-05)

dm3

Number of total reads

14057618

Reads aligned (%)

95.7

Duplicates removed (%)

13.5

Number of peaks

3241 (qval < 1E-05)