Antigen

Antigen Class

Histone

Antigen

H4K20me1

Cell type

Cell type Class

Cell line

Cell type

Kc167

Source

e/se

Developmental Stage

dorsal closure stage

Sample

source_name

Kc167

experiment

H4K20me1

chip antibody

Rabbi anti-H4K20me1 (Abcam, Egelhofer 2011)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In early origin experiments, chromatin was isolated and BrdU was immunoprecipted as described in (Ryba et al., Nat Protoc, 2011). H4K20me1 and γ-H2Av ChIP were performed as described in (MacAlpine et al., Genome Research, 2010). Sequencing libraries were prepared using Illumina's TruSeq protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

16265894

Reads aligned (%)

64.9

Duplicates removed (%)

34.5

Number of peaks

5340 (qval < 1E-05)

dm3

Number of total reads

16265894

Reads aligned (%)

65.0

Duplicates removed (%)

32.8

Number of peaks

6217 (qval < 1E-05)