Antigen

Antigen Class

TFs and others

Antigen

BrdU

Cell type

Cell type Class

Cell line

Cell type

Kc167

Source

e/se

Developmental Stage

dorsal closure stage

Sample

source_name

Kc167

experiment

early origin

chip antibody

Mouse anti-BrdU (BD Biosciences #555627)

RNAi treatment

Suv4-20

overexpression

PR-Set7

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In early origin experiments, chromatin was isolated and BrdU was immunoprecipted as described in (Ryba et al., Nat Protoc, 2011). H4K20me1 and γ-H2Av ChIP were performed as described in (MacAlpine et al., Genome Research, 2010). Sequencing libraries were prepared using Illumina's TruSeq protocols

Sequencing Platform

instrument_model

Illumina HiSeq 2000

dm6

Number of total reads

20007312

Reads aligned (%)

59.2

Duplicates removed (%)

44.6

Number of peaks

3102 (qval < 1E-05)

dm3

Number of total reads

20007312

Reads aligned (%)

61.2

Duplicates removed (%)

41.7

Number of peaks

2604 (qval < 1E-05)