Antigen

Antigen Class

TFs and others

Antigen

BrdU

Cell type

Cell type Class

Cell line

Cell type

Kc167

Source

e/se

Developmental Stage

dorsal closure stage

Sample

source_name

Kc167

experiment

early origin

chip antibody

Mouse anti-BrdU (BD Biosciences #555627)

RNAi treatment

ATR & PR-Set7

replicate

2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

In early origin experiments, chromatin was isolated and BrdU was immunoprecipted as described in (Ryba et al., Nat Protoc, 2011). H4K20me1 and γ-H2Av ChIP were performed as described in (MacAlpine et al., Genome Research, 2010). Sequencing libraries were prepared using Illumina's TruSeq protocols

Sequencing Platform

instrument_model

Illumina MiSeq

dm6

Number of total reads

1585104

Reads aligned (%)

72.4

Duplicates removed (%)

30.8

Number of peaks

764 (qval < 1E-05)

dm3

Number of total reads

1585104

Reads aligned (%)

73.9

Duplicates removed (%)

23.3

Number of peaks

918 (qval < 1E-05)