Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
BrdU

Cell type

Cell type Class
Cell line
Cell type
Kc167
Source
e/se
Developmental Stage
dorsal closure stage

Attributes by original data submitter

Sample

source_name
Kc167
experiment
early origin
chip antibody
Mouse anti-BrdU (BD Biosciences #555627)
RNAi treatment
PR-Set7

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
In early origin experiments, chromatin was isolated and BrdU was immunoprecipted as described in (Ryba et al., Nat Protoc, 2011). H4K20me1 and γ-H2Av ChIP were performed as described in (MacAlpine et al., Genome Research, 2010). Sequencing libraries were prepared using Illumina's TruSeq protocols

Sequencing Platform

instrument_model
Illumina MiSeq

dm6

Number of total reads
1858993
Reads aligned (%)
71.9
Duplicates removed (%)
39.3
Number of peaks
424 (qval < 1E-05)

dm3

Number of total reads
1858993
Reads aligned (%)
73.7
Duplicates removed (%)
32.4
Number of peaks
894 (qval < 1E-05)

Base call quality data from DBCLS SRA