GSM1898101: 82-kDa ChAT (A-beta); Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
CHAT
Cell type
Cell type Class
Neural
Cell type
SH-SY5Y
Primary Tissue
Brain
Tissue Diagnosis
Neuroblastoma
Attributes by original data submitter
Sample
source_name
SH-SY5Y cells
cell line
SH-SY5Y
cell type
neural cells
passages
8-10
treated with
100 nM amyloid-beta1-42 oligomers for 4 h
chip antibody
In-house C-terminal anti-ChAT antibody
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with paraformaldehyde, lysed and sonicated prior to extraction of DNA-protein complexes with antibody ChIP-seq samples were individually barcoded with Illumina TruSeq® adapter sequences for library construction at the Donnelly Sequencing Centre (Toronto, ON) according to the manufacturer's instructions