Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K36me2

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4-11 human leukemia cells
cell line
MV4-11
antibody
H3K36me2 (Active Motif 39255)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole genome ChIP using MV4-11 cells was performed under conditions described previously (Boyer et al., 2005) using 2-4×107 cells per IP with specific antibodies ChIP-seq libraries were prepared according to the Illumina protocol and sequenced using Illumina HiSeq. Sequencing was carried out at the Stanford Genome Center using Illumina HiSeq 2000. The read length is 50bp.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
35072966
Reads aligned (%)
98.3
Duplicates removed (%)
5.2
Number of peaks
65340 (qval < 1E-05)

hg19

Number of total reads
35072966
Reads aligned (%)
98.0
Duplicates removed (%)
5.5
Number of peaks
69736 (qval < 1E-05)

Base call quality data from DBCLS SRA