Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ASH1L

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4-11 human leukemia cells
cell line
MV4-11
antibody
ASH1L (Bethyl Laboratories A301-749A)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole genome ChIP using MV4-11 cells was performed under conditions described previously (Boyer et al., 2005) using 2-4×107 cells per IP with specific antibodies ChIP-seq libraries were prepared according to the Illumina protocol and sequenced using Illumina HiSeq. Sequencing was carried out at the Stanford Genome Center using Illumina HiSeq 2000. The read length is 50bp.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
65673919
Reads aligned (%)
56.3
Duplicates removed (%)
35.6
Number of peaks
1283 (qval < 1E-05)

hg38

Number of total reads
65673919
Reads aligned (%)
58.5
Duplicates removed (%)
33.7
Number of peaks
2153 (qval < 1E-05)

Base call quality data from DBCLS SRA