Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
AFF1

Cell type

Cell type Class
Blood
Cell type
MV-4-11
Primary Tissue
Blood
Tissue Diagnosis
Leukemia Acute Myelogenous

Attributes by original data submitter

Sample

source_name
MV4-11 human leukemia cells
cell line
MV4-11
antibody
AF4 (Michael Cleary Lab C2-1)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Whole genome ChIP using MV4-11 cells was performed under conditions described previously (Boyer et al., 2005) using 2-4×107 cells per IP with specific antibodies ChIP-seq libraries were prepared according to the Illumina protocol and sequenced using Illumina HiSeq. Sequencing was carried out at the Stanford Genome Center using Illumina HiSeq 2000. The read length is 50bp.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
45328252
Reads aligned (%)
88.5
Duplicates removed (%)
15.7
Number of peaks
1866 (qval < 1E-05)

hg19

Number of total reads
45328252
Reads aligned (%)
87.7
Duplicates removed (%)
17.5
Number of peaks
1230 (qval < 1E-05)

Base call quality data from DBCLS SRA