Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Mef2c

Cell type

Cell type Class
Blood
Cell type
B cells
NA
NA

Attributes by original data submitter

Sample

source_name
BMiFLT3 (15-3) cells
cell line
BMiFLT3(15-3)
cell type
proB cells from a murine BCP-ALL
chip antibody
anti-Mef2c (Santa Cruz; catalog# sc-13266X; lot# C2309)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
ChIP experiments using anti-Mef2c (Santa Cruz; sc-13266X; Lot#C2309) or anti-IgG (Santa Cruz; sc-2028; Lot#J2114) antibodies were performed using the EZ-ChIP Kit (Upstate/Millipore) according to the manufacturer’s protocol. Purified DNA from chromatin immunoprecipitations (10-50 ng) was adapter-ligated using the Illumina-compatible NEXTflexTM ChIP Seq-Kit (Bioo Scientific) for DNA inserts ≥70 bps.DNA fragments between 350-550 bp were isolated using PippinPrep (Sage Science) and quantified and sized on a microfluidics-based Bioanalyzer 2100 using a High Sensitivity DNA Kit (Agilent).

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
39269937
Reads aligned (%)
97.8
Duplicates removed (%)
20.8
Number of peaks
507 (qval < 1E-05)

mm9

Number of total reads
39269937
Reads aligned (%)
97.4
Duplicates removed (%)
20.7
Number of peaks
581 (qval < 1E-05)

Base call quality data from DBCLS SRA