Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
5-mC

Cell type

Cell type Class
Embryonic fibroblast
Cell type
3T3-L1
Tissue
Embryo
Cell Type
Fibroblast

Attributes by original data submitter

Sample

source_name
3T3-L1 preadipocytes
cell type
3T3-L1 preadipocytes
ChIP
m5CpG
day
Day 0
sirna/shrna
-

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Sonicated nuclear fraction was incubated with each antibody and histone-DNA or SETDB1-DNA complexes were isolated. Sonicated DNA was incubated with MBD domain of MBD1 and m5CpG was isolated. Libraries were prepared according to Illumina's instructions.

Sequencing Platform

instrument_model
Illumina Genome Analyzer IIx

mm10

Number of total reads
37409594
Reads aligned (%)
95.1
Duplicates removed (%)
55.3
Number of peaks
125259 (qval < 1E-05)

mm9

Number of total reads
37409594
Reads aligned (%)
94.3
Duplicates removed (%)
55.2
Number of peaks
125254 (qval < 1E-05)

Base call quality data from DBCLS SRA