Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Blood

Cell type

Treg

NA

NA

Sample

source_name

CD4+ T cells

antibody

H3K27ac, Abcam, ab4729

cell source

Primary Human T cells isolated from buffy coat preparations and sorted

cell type

activated Treg cells (CD4+ CD25hi CD45RO+) from donor 21

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

DNA was extracted by ChIP-seq Standard Illumina library prep

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

71596244

Reads aligned (%)

76.2

Duplicates removed (%)

78.1

Number of peaks

3147 (qval < 1E-05)

hg19

Number of total reads

71596244

Reads aligned (%)

75.5

Duplicates removed (%)

79.3

Number of peaks

3124 (qval < 1E-05)