Antigen

Antigen Class

Unclassified

Antigen

Unclassified

Cell type

Cell type Class

Breast

Cell type

MCF-7

Primary Tissue

Breast

Site of Extraction

Pleura

Tissue Diagnosis

Adenocarcinoma

Sample

source_name

MCF7-xenograft

tissue

Snap-frozen tissue

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIPseq:ThruPLEX-FD kits (Rubicon Genomics) was used and 75 bp single-end reads were sequenced on a NextSeq 500 instrument (Illumina). For RNAseq: TruSeq RNA Sample Preparation Kit (Illumina) was used according to the manufacturer's instructions and 75 bp single-end reads were sequenced on an Illumina NextSeq 500 instrument.

Sequencing Platform

instrument_model

NextSeq 500

hg38

Number of total reads

31237397

Reads aligned (%)

71.0

Duplicates removed (%)

1.1

Number of peaks

448 (qval < 1E-05)

hg19

Number of total reads

31237397

Reads aligned (%)

70.5

Duplicates removed (%)

1.3

Number of peaks

409 (qval < 1E-05)