GSM1891979: Rib Chondrocyte Jun ChIPseq; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
Jun
Cell type
Cell type Class
Bone
Cell type
Chondrocytes
MeSH Description
Polymorphic cells that form cartilage.
Attributes by original data submitter
Sample
source_name
p1 mouse rib chondrocytes
cell type
chondrocytes
tissue
rib
developmental stage
p1
strain
ICR mice
chip antibody
Jun(Abcam Cat#ab31419)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
postnatal day 1 (p1) rib chondrocytes isolated from wild-type ICR mice were immediately cross-linked with 1% formaldehyde for 10 minutes at room temperature, then 0.125 M Glycine was added to quench formaldehyde. 20,000,000 cells were used for a ChIP reaction. Chromatin was sonicated by 10 sessions of 30 pulses (1 sec on and 1 sec off) at 50% amplitude using the Branson sonifier 250D (Branson Ultrasonics Corporation, Danbury, CT) in order to obtain 100 – 600 bp of DNA fragments. One hundred microlitters of Dynabeads M-280 Sheep anti-Rabbit IgG was combined with 9 μg of antibodies for Jun. ChIPseq libraries were constructed with input control samples (input DNA) using ChIP-seq DNA Sample Prep Kit (IP-102-1001; Illumina Inc., San Diego, CA), according to manufacturer’s instruction. Adaptor-modified DNA fragments were enriched by 18 cycles of PCR.