Antigen

Antigen Class

Histone

Antigen

H3K27ac

Cell type

Cell type Class

Digestive tract

Cell type

HCT 116

Primary Tissue

Colon

Tissue Diagnosis

Carcinoma

Sample

source_name

HCT 116

cimp status

CIMP (+)

chip antibody

H3K27ac

cell line

HCT 116

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cells were fixed with 1% formaldehyde for 15 minutes (Brd4 ChIP) or 5 minutes (H3K27ac ChIP). DNA was sonicated to an average length of 200 - 500 bp. IP and Input DNA was reverse crosslinked and purified for library preparation. Illumina sequencing libraries were prepared from the ChIP and Input DNAs by the standard consecutive enzymatic steps of end-polishing, dA-addition, and adaptor ligation.

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

41002861

Reads aligned (%)

98.8

Duplicates removed (%)

11.6

Number of peaks

46070 (qval < 1E-05)

hg19

Number of total reads

41002861

Reads aligned (%)

98.5

Duplicates removed (%)

12.0

Number of peaks

45991 (qval < 1E-05)