Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA

Attributes by original data submitter

Sample

source_name
CD8 T cells
genotype
wild type
tissue
spleen
cell type
T cells
cell phenotype
TCRbeta high, CD8+
chip antibody
None

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenic CD8 T cells were sort-purified from WT or Tcf1/Lef1-deficient mice, the cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with antibodies against Sin3A, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
19309156
Reads aligned (%)
95.7
Duplicates removed (%)
6.6
Number of peaks
517 (qval < 1E-05)

mm9

Number of total reads
19309156
Reads aligned (%)
95.6
Duplicates removed (%)
6.7
Number of peaks
551 (qval < 1E-05)

Base call quality data from DBCLS SRA