GSM1889270: WT CD8 Input for mSin3A; Mus musculus; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Input control
Antigen
Input control
Cell type
Cell type Class
Blood
Cell type
CD8+ T cells
NA
NA
Attributes by original data submitter
Sample
source_name
CD8 T cells
genotype
wild type
tissue
spleen
cell type
T cells
cell phenotype
TCRbeta high, CD8+
chip antibody
None
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Splenic CD8 T cells were sort-purified from WT or Tcf1/Lef1-deficient mice, the cells were then fixed and sonicated to generate chromatin fragments, followed by immunoprecipitation with antibodies against Sin3A, which were then properly washed and immunoprecipitated DNA extracted. The DNA segments from ChIP were end-repaired and ligated to indexed Illumina adaptors, followed by amplification by PCR with a low number of cells (18-21 cycles). The resulting libraries were seqeunced with Illumina Hiseq-2000 platform.