Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
MEF
Tissue
Embryonic Fibroblast
Lineage
primaryCells
Description
Mouse Embryonic Fibroblast

Attributes by original data submitter

Sample

source_name
IgG.FC/FC
cell type
Immortalized Mouse Embryonic Fibroblast (MEF) cells in which both Mll2 alleles had been targeted by loxP (F/F cells)
treatment
24 hours exposure to tamoxifen
resulting cell type
MLL2 FC/FC MEF cells
chip antibody
anti-IgG (Sigma, catalog# M8642)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cells were cross-linked with 1% formaldehyde. Nuclei were extracted in low salt conditions and burst in high salt before shearing the DNA to reduce the size to ~400 bp and performing ChIP with the appropriate antibodies. DNA libraries were prepared according to Illumina's standard instructions.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
44517893
Reads aligned (%)
65.2
Duplicates removed (%)
71.8
Number of peaks
554 (qval < 1E-05)

mm9

Number of total reads
44517893
Reads aligned (%)
65.0
Duplicates removed (%)
71.9
Number of peaks
565 (qval < 1E-05)

Base call quality data from DBCLS SRA