Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Embryonic fibroblast
Cell type
NIH/3T3
Primary Tissue
Embryo
Tissue Diagnosis
Normal

Attributes by original data submitter

Sample

source_name
embryonic fibroblasts, input
cell line
NIH 3T3
cell type
embryonic fibroblast
treatment
none
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was prepared from sonicated nuclei and specific protein-DNA complexes were isolated by immunoprecipitation. Libraries for Illumina sequencing were prepared using the TruSeq ChIP Sample Prep kit (Illumina) for the input chromatin samples.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
50341566
Reads aligned (%)
97.6
Duplicates removed (%)
16.6
Number of peaks
571 (qval < 1E-05)

mm9

Number of total reads
50341566
Reads aligned (%)
97.2
Duplicates removed (%)
16.5
Number of peaks
649 (qval < 1E-05)

Base call quality data from DBCLS SRA