GSM1876190: MCF7 Input DNA; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Unclassified
Antigen
Unclassified
Cell type
Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma
Attributes by original data submitter
Sample
source_name
Breast Cancer Cell Line
gender
female
cell line
MCF7
er status
ER+
confluency
Low
mi63 treatment
No
foxm1 antibody chip
No
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Harvested MCF-7 cells were fixed with 1% formaldehyde for 15 min and quenched with 0.125 M glycine. Lysates were clarified from sonicated nuclei and FOXM1-DNA complexes were isolated with antibody (Santa Cruz, sc-501, Lot. D0110). ChIP and Input DNAs were prepared for amplification by converting overhangs into phosphorylated blunt ends and adding an adenine to the 3’-ends. Illumina genomic adapters were ligated and the sample was size-fractionated (200-250 bp) on a 2% agarose gel. After a final PCR amplification step (18 cycles), the resulting DNA libraries were quantified and sequenced on Illumina GAII (36 nt reads, single-end).