ChIP-Atlas: SRX1225222

Antigen

Cell type Class: Bone
Cell type: Osteoclasts
MeSH Description: A large multinuclear cell associated with the BONE RESORPTION. An odontoclast, also called cementoclast, is cytomorphologically the same as an osteoclast and is involved in CEMENTUM resorption.

source_name: Primary mouse OCP cells (Anet al. 2014 J Biol Chem 289: 24366-24373)
cell type: Primary mouse osteoclast cells
strain: C57BL/6 mice
age: 0 d
chip antibody: input

library_strategy: ChIP-Seq
library_source: GENOMIC
library_selection: ChIP
library_construction_protocol: After treatment with M-CSF, floating cells were removed and adherent cells were used as bone marrow-derived macrophage (BMM) cells. On day 3, the cells were stained for tartrate-resistant acid phosphatase (TRAP). The TRAP-positive multinucleated cells containing three or more nuclei are referred as osteoclasts.Chromatin was acetylated with acetic anhydride (20 mM final). cetylated chromatin was immunoprecipitated with an H3K14ac-specific antibody (Active Motif) or an H3 C-terminal (H3CT) antibody (Abcam). Libraries were prepared according to Illumina's instructions accompanying the DNA Sample Kit. Briefly, DNA was end-repaired and blunt, phosphorylated ends were treated with Klenow fragment and dATP to yield a protruding 3- 'A' base for ligation of Illumina's adapters. After adapter ligation DNA was PCR amplified with Illumina primers for 15 cycles and library fragments of ~250 bp (insert plus adaptor and PCR primer sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation. Libraries were sequenced on the Illumina Hi-Seq following the manufacturer's protocols.sequences) were band isolated from an agarose gel. The purified DNA was captured on an Illumina flow cell for cluster generation.