Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
LEF1

Cell type

Cell type Class
Uterus
Cell type
RL95-2
Primary Tissue
Uterus
Tissue Diagnosis
Carcinoma

Attributes by original data submitter

Sample

source_name
RL95-2 cells
antibody
LEF2
egf hr
12hr
cell line
RL95-2

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Immunoprecipitation was carried out using antibodies against EpCAM and Lef-1 on SX-8G-Star Compact Automated System following the manufacturer’s instructions. Pull-down products were purified and checked for size distribution for quality control by 2100 Bioanalyzer. Library construction and sequencing were performed in the Genome Sequencing Facility in University of Texas Health Science Center at San Antonio by HiSeq 2000 sequencing system under the guidelines.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
46988465
Reads aligned (%)
86.9
Duplicates removed (%)
7.8
Number of peaks
1205 (qval < 1E-05)

hg38

Number of total reads
46988465
Reads aligned (%)
88.9
Duplicates removed (%)
6.7
Number of peaks
1156 (qval < 1E-05)

Base call quality data from DBCLS SRA