Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Embryonic fibroblast

Cell type

MEF

Tissue

Embryonic Fibroblast

Lineage

primaryCells

Description

Mouse Embryonic Fibroblast

Sample

source_name

Mouse Embryonic Fibroblast(MEF)_wildtype_gluscose_starvation_input

strain

C57BL/6

passages

3 to 5

cell type

Embryonic Fibroblast

genotype

wildtype

chip antibody

none (input)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

For ChIP experiments, cell lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with H3R17me2 antibody. ChIP-seq libraries were prepared for sequencing using the NEXTflex ChIP-seq kit, according to the manufacturer's instructions

Sequencing Platform

instrument_model

Illumina HiSeq 2500

mm10

Number of total reads

27037181

Reads aligned (%)

98.4

Duplicates removed (%)

15.6

Number of peaks

490 (qval < 1E-05)

mm9

Number of total reads

27037181

Reads aligned (%)

98.1

Duplicates removed (%)

15.6

Number of peaks

525 (qval < 1E-05)