Antigen

Antigen Class

Input control

Antigen

Input control

Cell type

Cell type Class

Bone

Cell type

Osteosarcoma cell lines

NA

NA

Sample

source_name

SCOS#2 Dox ON 72h_input

cell line

SCOS#2

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and histone-DNA complexes were isolated with antibody. We used anti HA antibody(Monoclonal Mouse IgG 1-κ):Nacalai cat# 06340-54 Lot#M4H0340. Libraries were prepared according to illumina'sTrueseq ChIP sample prep kit set A(Part#IP-202-1012)

Sequencing Platform

instrument_model

Illumina MiSeq

mm10

Number of total reads

7814934

Reads aligned (%)

98.5

Duplicates removed (%)

12.3

Number of peaks

137 (qval < 1E-05)

mm9

Number of total reads

7814934

Reads aligned (%)

98.4

Duplicates removed (%)

12.5

Number of peaks

98 (qval < 1E-05)