Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
TCF3

Cell type

Cell type Class
Blood
Cell type
RAMOS
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type

Attributes by original data submitter

Sample

source_name
Human Ramos cell line
cell line
Ramos
chip antibody
anti-E2A (sc-349; Santa Cruz Technologies)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20 million cells from the human Ramos cell line were crosslinked by the addition of 1% formaldehyde at room temperature for 10 min and quenched with 0.25M glycine followed by sonication and immunoprecipitation with 10 μg of anti-E2A (sc-349; Santa Cruz Technologies). DNA fragments were blunt-end ligated to the Illumina adaptors, amplified, and sequenced with an Illumina HiSeq Sequencer.

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
42961428
Reads aligned (%)
89.3
Duplicates removed (%)
5.1
Number of peaks
1861 (qval < 1E-05)

hg38

Number of total reads
42961428
Reads aligned (%)
91.2
Duplicates removed (%)
3.8
Number of peaks
1849 (qval < 1E-05)

Base call quality data from DBCLS SRA