GSM1873237: ChIP-seq for transcription factor E2A; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
TCF3
Cell type
Cell type Class
Blood
Cell type
RAMOS
Primary Tissue
Blood
Tissue Diagnosis
Malignant Lymphoma - Burkitts Type
Attributes by original data submitter
Sample
source_name
Human Ramos cell line
cell line
Ramos
chip antibody
anti-E2A (sc-349; Santa Cruz Technologies)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
20 million cells from the human Ramos cell line were crosslinked by the addition of 1% formaldehyde at room temperature for 10 min and quenched with 0.25M glycine followed by sonication and immunoprecipitation with 10 μg of anti-E2A (sc-349; Santa Cruz Technologies). DNA fragments were blunt-end ligated to the Illumina adaptors, amplified, and sequenced with an Illumina HiSeq Sequencer.