GSM1871946: H3K4me3 ChIPSeq NCCIT 10k; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
Histone
Antigen
H3K4me3
Cell type
Cell type Class
Pluripotent stem cell
Cell type
NCCIT
Primary Tissue
Testis
Tissue Diagnosis
Teratoma
Attributes by original data submitter
Sample
source_name
NCCIT (ATCC® CRL-2073)
cell line
NCCIT (ATCC CRL-2073)
chip antibody
H3K4me3 (Merck Millipore, catalogue number 04-745)
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Chromatin was preapred from whole cell lysates of cross-linked cells. Lysates were sonicated and chromatin complexes were isolated with antibodies coupled to magnetic beads. Libraries were prepared according to Rubicon Genomics THRUPlex kit with minor modifications.