Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Breast
Cell type
Mammary glands
NA
NA

Attributes by original data submitter

Sample

source_name
Mammary
strain
C57BL/6
tissue
Mammary gland
state
lactation day of 1
genotype
wild type
chip antibody
none

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Frozen-stored mammary tissues from L1 or p13 were broken into powder with mortar and pestle. Nuclei were extracted in Farnham lysis buffer (5mM PIPES pH8.0, 85mM KCl, 0.5% NP-40 and protease inhibitors). Antibodies against H3K27ac (Abcam, Ab4729), MED1 (Bethyl laboratory, A300-793A), RNA Pol II (Abcam, Ab5408), GR (Thermo Scientific, PA1-511A) were used for ChIP. Libraries were prepared for sequencing using standard Illumina protocols.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
30383554
Reads aligned (%)
91.4
Duplicates removed (%)
9.5
Number of peaks
252 (qval < 1E-05)

mm9

Number of total reads
30383554
Reads aligned (%)
91.3
Duplicates removed (%)
9.5
Number of peaks
265 (qval < 1E-05)

Base call quality data from DBCLS SRA