Antigen

Antigen Class

Histone

Antigen

H3K4me3

Cell type

Cell type Class

Prostate

Cell type

C4-2

Tissue

Prostate

Cell Type

Epithelial

Disease

Prostate Cancer

Sample

source_name

C4-2B cells cultured in the presence of SR2211 (5 µM)

cell line

C4-2B

cell type

human prostate cancer cell line

treated with

5 µM SR2211 for 24hrs

chip antibody

H3K4me3 (Abcam; ab8580)

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's paired-end DNA sample Prep kit.

Sequencing Platform

instrument_model

Illumina HiSeq 2000

hg38

Number of total reads

37481783

Reads aligned (%)

98.9

Duplicates removed (%)

19.5

Number of peaks

26448 (qval < 1E-05)

hg19

Number of total reads

37481783

Reads aligned (%)

98.2

Duplicates removed (%)

21.2

Number of peaks

26161 (qval < 1E-05)