Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Histone
Antigen
H3K4me3

Cell type

Cell type Class
Prostate
Cell type
C4-2
Tissue
Prostate
Cell Type
Epithelial
Disease
Prostate Cancer

Attributes by original data submitter

Sample

source_name
C4-2B cells cultured in medium with vehicle control
cell line
C4-2B
cell type
human prostate cancer cell line
treated with
vehicle for 24hrs
chip antibody
H3K4me3 (Abcam; ab8580)

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and protein-DNA complexes were isolated with antibody. Libraries were prepared according to Illumina's paired-end DNA sample Prep kit.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
42083546
Reads aligned (%)
99.0
Duplicates removed (%)
18.2
Number of peaks
25814 (qval < 1E-05)

hg19

Number of total reads
42083546
Reads aligned (%)
98.2
Duplicates removed (%)
20.2
Number of peaks
25453 (qval < 1E-05)

Base call quality data from DBCLS SRA