Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
CBP

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cell
chip antibody
anti-CBP, custom-made antibodies

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from Mnase digested nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using TruSeq ChIP Sample Prep Kit (Illumina) according to the manufacture’s instruction.

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
22183245
Reads aligned (%)
127.2
Duplicates removed (%)
34.9
Number of peaks
6410 (qval < 1E-05)

dm6

Number of total reads
22183245
Reads aligned (%)
124.2
Duplicates removed (%)
36.3
Number of peaks
6001 (qval < 1E-05)

Base call quality data from DBCLS SRA