Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
Etl1

Cell type

Cell type Class
Cell line
Cell type
S2
Source
Oregon R
Developmental Stage
late embryonic stage

Attributes by original data submitter

Sample

source_name
S2 cell
chip antibody
anti-SMARCAD1 (ASNA), custom-made antibodies

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from Mnase digested nuclei and histone-DNA complexes were isolated with antibody. ChIP-seq libraries were prepared using TruSeq ChIP Sample Prep Kit (Illumina) according to the manufacture’s instruction.

Sequencing Platform

instrument_model
Illumina MiSeq

dm3

Number of total reads
20484019
Reads aligned (%)
97.5
Duplicates removed (%)
42.6
Number of peaks
4636 (qval < 1E-05)

dm6

Number of total reads
20484019
Reads aligned (%)
97.2
Duplicates removed (%)
43.6
Number of peaks
4449 (qval < 1E-05)

Base call quality data from DBCLS SRA