Sample information curated by ChIP-Atlas

Antigen

Antigen Class
Input control
Antigen
Input control

Cell type

Cell type Class
Epidermis
Cell type
Keratinocytes
MeSH Description
Epidermal cells which synthesize keratin and undergo characteristic changes as they move upward from the basal layers of the epidermis to the cornified (horny) layer of the skin. Successive stages of differentiation of the keratinocytes forming the epidermal layers are basal cell, spinous or prickle cell, and the granular cell.

Attributes by original data submitter

Sample

source_name
Primary keratinocytes trancduced with AhRCA-HA
chip-antibody
none
strain
ICR
cell type
Primary keratinocytes

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei, and protein-DNA complexes were isolated using antibody to HA and Dynabeads-proteinA and -proteinG Libraries were prepared according to NuGEN's instructions accompanying the Ovation Ultralow DR Multiplex System 1-8 (NuGEN, 0330-32).

Sequencing Platform

instrument_model
Illumina HiSeq 2000

mm10

Number of total reads
33605063
Reads aligned (%)
95.5
Duplicates removed (%)
9.3
Number of peaks
239 (qval < 1E-05)

mm9

Number of total reads
33605063
Reads aligned (%)
95.5
Duplicates removed (%)
9.3
Number of peaks
216 (qval < 1E-05)

Base call quality data from DBCLS SRA