Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
RAD21

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF7 cells
cell line
MFC7
cell type
Breast adenocarcinoma
chip antibody
RAD21, abcam, ab992

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. Biological replicates from each experiment were completed. For RNA-seq polyA RNA was isolated using oligo dT beads, and libraries prepared. Illumina Single End

Sequencing Platform

instrument_model
Illumina HiSeq 2500

hg19

Number of total reads
23334051
Reads aligned (%)
85.1
Duplicates removed (%)
7.6
Number of peaks
43867 (qval < 1E-05)

hg38

Number of total reads
23334051
Reads aligned (%)
86.3
Duplicates removed (%)
6.8
Number of peaks
43888 (qval < 1E-05)

Base call quality data from DBCLS SRA