Antigen

Antigen Class

TFs and others

Antigen

CREB1

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2 cells

cell line

HepG2

cell type

Hepatocellular Carcinoma

chip antibody

CREB1 Santa Cruz Biotechnology, sc-240

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. Biological replicates from each experiment were completed. For RNA-seq polyA RNA was isolated using oligo dT beads, and libraries prepared. Illumina Single End

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg38

Number of total reads

32557166

Reads aligned (%)

84.7

Duplicates removed (%)

14.1

Number of peaks

36076 (qval < 1E-05)

hg19

Number of total reads

32557166

Reads aligned (%)

84.4

Duplicates removed (%)

14.4

Number of peaks

36855 (qval < 1E-05)