Antigen

Antigen Class

TFs and others

Antigen

RAD21

Cell type

Cell type Class

Liver

Cell type

Hep G2

Primary Tissue

Liver

Tissue Diagnosis

Carcinoma Hepatocellular

Sample

source_name

HepG2 cells

cell line

HepG2

cell type

Hepatocellular Carcinoma

chip antibody

RAD21, abcam, ab992

Sequenced DNA Library

library_strategy

ChIP-Seq

library_source

GENOMIC

library_selection

ChIP

library_construction_protocol

Cross-linked chromatin was immunoprecipitated with an antibody. The Protein:DNA crosslinks were then reversed and the DNA fragments were recovered and sequenced. Please see protocol notes below and go to http://hudsonalpha.org/myers-lab/protocols for the most current version of the protocol. Biological replicates from each experiment were completed. For RNA-seq polyA RNA was isolated using oligo dT beads, and libraries prepared. Illumina Single End

Sequencing Platform

instrument_model

Illumina HiSeq 2500

hg19

Number of total reads

19087095

Reads aligned (%)

95.8

Duplicates removed (%)

9.1

Number of peaks

47071 (qval < 1E-05)

hg38

Number of total reads

19087095

Reads aligned (%)

96.2

Duplicates removed (%)

8.7

Number of peaks

47153 (qval < 1E-05)