Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
NR3C1

Cell type

Cell type Class
Breast
Cell type
T-47D
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
T-47D breast cancer cell line
cell line
T-47D
cell type
Pleural effusion from a ductal carcinoma of the breast
treatment
Dexamethasone
concentration
100nM
time
30 minutes
replicate
replicate 2
antibody
GR E-20X sc-1003 Santa Cruz
growth proptocol
For maintenance cells were cultured in RPMI containing 2mM L-glutamine supplemented 10% calf serum, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
32270775
Reads aligned (%)
88.6
Duplicates removed (%)
7.8
Number of peaks
1525 (qval < 1E-05)

hg38

Number of total reads
32270775
Reads aligned (%)
91.2
Duplicates removed (%)
5.9
Number of peaks
1844 (qval < 1E-05)

Base call quality data from DBCLS SRA