Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
ESR1

Cell type

Cell type Class
Breast
Cell type
ZR-75-1
Primary Tissue
Breast
Tissue Diagnosis
Adenocarcinoma Ductal

Attributes by original data submitter

Sample

source_name
ZR-75-1 breast cancer cell line
cell line
ZR-75-1
cell type
Ascites from a ductal carcinoma of the breast
treatment
untreated
time
30 minutes
replicate
replicate 2
antibody
ER cocktail: Ab-10 Thermo Scientific Lab Vision, HC-20 sc-543 Santa Cruz
growth proptocol
For maintenance cells were cultured in RPMI containing 2mM L-glutamine supplemented 10% calf serum, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg19

Number of total reads
26230524
Reads aligned (%)
78.9
Duplicates removed (%)
17.2
Number of peaks
17921 (qval < 1E-05)

hg38

Number of total reads
26230524
Reads aligned (%)
81.1
Duplicates removed (%)
15.6
Number of peaks
18025 (qval < 1E-05)

Base call quality data from DBCLS SRA