Sample information curated by ChIP-Atlas

Antigen

Antigen Class
TFs and others
Antigen
FOXA1

Cell type

Cell type Class
Breast
Cell type
MCF-7
Primary Tissue
Breast
Site of Extraction
Pleura
Tissue Diagnosis
Adenocarcinoma

Attributes by original data submitter

Sample

source_name
MCF-7 breast cancer cell line
cell line
MCF-7
cell type
Pleural effusion from an adenocarcinoma of the breast
treatment
untreated
time
30 minutes
replicate
replicate 1
antibody
FoxA1 ab23738 Abcam
growth proptocol
For maintenance cells were cultured in DMEM supplemented 10% calf serum, 2mM L-glutamine, 1mM sodium pyruvate, 1X non-essential amino acids, and 1% penicillin-streptomycin

Sequenced DNA Library

library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Lysates were clarified from sonicated nuclei and GR/DNA, ER/DNA, of FoxA1/DNA complexes were isolated with antibody.

Sequencing Platform

instrument_model
Illumina HiSeq 2000

hg38

Number of total reads
32815041
Reads aligned (%)
92.7
Duplicates removed (%)
14.3
Number of peaks
40273 (qval < 1E-05)

hg19

Number of total reads
32815041
Reads aligned (%)
92.3
Duplicates removed (%)
14.9
Number of peaks
40197 (qval < 1E-05)

Base call quality data from DBCLS SRA